Neohalo Bioscience makes its debut at the Life Science New Product Investment Promotion Conference.

On the grand symphony of life, small RNAs are undoubtedly an indispensable part. Small RNAs refer to non-coding RNAs with a length of less than 200 nucleotides, including miRNA, siRNA, piRNA, snRNA, etc. Small RNAs widely exist in living organisms and are an important class of functional molecules. They are tiny and delicate, yet they play a crucial role inside cells, regulating gene expression and participating in various life activities.

Research has found that small RNAs play an important role in the pathological process of tumors. They can induce the instability of target gene expression and inhibit the proliferation and metastasis of tumor cells. In the nervous system, small RNAs also play a key regulatory role, affecting the growth, differentiation, and function of nerve cells. With the continuous progress of technology, our understanding of small RNAs is constantly deepening. High-throughput sequencing technology has the advantages of complete detection, high accuracy, good reproducibility, and high throughput, and thus has great advantages in exploring and researching small RNAs. Of course, if one wants to carry out high-throughput sequencing experiments, the processes of sample extraction and library construction are essential.

Today, let's embark on the amazing journey of small RNA extraction and library construction together and explore the biological functions of these tiny molecules in depth. First of all, let's talk about the extraction of small RNAs. This process is like screening out the shining pearls from the ocean of life. Scientists carefully extract these precious small RNA molecules from the complex cellular environment with elaborate experimental designs and professional reagents.

The plasma/serum small RNA extraction kit independently developed by Neohalo Bioscience has a lysis buffer that can effectively disrupt the components with cell membrane structures in the samples and denature proteins to release nucleic acids. Meanwhile, it uses the Silica-Magnetic Aggregates with a patented structure (SMAGG) to adsorb small RNAs. And by taking advantage of the reversible magnetic response physical property of SMAGG, it enriches and washes away the non-specifically bound impurities and salts. Finally, the purified small RNAs are separated from the magnetic microspheres using an elution buffer, obtaining small RNAs that meet the requirements of downstream detection.

Next comes the crucial step of library construction. Library construction is like building a home for these small RNAs, allowing them to live together in an orderly manner. Scientists utilize high-throughput sequencing technology to amplify and label the extracted small RNAs, thereby constructing a library that contains a wealth of small RNA information. This library is like an encyclopedia of life, recording the types, quantities, and functions of small RNAs.

The library construction kit of Xinhui Biology adopts the unique patented technology for removing adapter self-ligation products, enabling the library construction of trace small RNA samples. Meanwhile, the selection and use of a one-tube reaction process further reduce the input amount of the starting template for library construction.

The small RNA library construction kit developed by Neohalo Bioscience is applicable to small RNA samples from different sources with an initial amount ranging from 500 pg to 20 ng. Through adapter ligation, reverse transcription, second-strand synthesis, and PCR amplification, the samples are finally transformed into libraries suitable for sequencing on Illumina and MGI platforms after library construction. According to experimental tests, the library yield and the number of detected miRNA species of the Xinhui kit are far higher than those of domestic and foreign competing products. At the same time, it has also been verified that the performance of the reagents between batches is stable.

In this wonderful journey of small RNA extraction and library construction, we can not only appreciate the charm of life sciences but also feel the power of science. By studying small RNAs, we can conduct more in-depth research on the functions of these tiny molecules, reveal their associations with life activities, and provide new ideas and directions for future medical research and treatment strategies. Let's look forward to this journey being able to continue to go deeper, uncovering more mysteries of life for mankind, and bringing more breakthroughs and surprises to human health and well-being!

Hangzhou Neohalo Biotechnology Co., Ltd. was established in April 2022. It is an upstream reagent and instrument platform supplier for nucleic acid research and is located in the China (Zhejiang) Pilot Free Trade Zone. NeohaloBioscience focuses on the development and promotion of nanomaterial and biochip products, and serves public health and life sciences with these. We are particularly committed to researching and developing internationally leading cfRNA detection-related technologies and products. At present, we have launched a trace small RNA isolation and purification kit based on the patented Silica-Magnetic Aggregates (SMAGG) technology and a trace small RNA library construction kit based on the patented technology for removing adapter self-ligation products. NeohaloBioscience's business is currently in a stage of rapid development. At this Distrimap investment promotion conference, NeohaloBioscience hopes to join hands with like-minded agent friends across the country to enable the vast number of Chinese scientific researchers to obtain a full-process solution for small RNA extraction and library construction that meets their needs.

Conference Name: Life Science New Product (Investment Promotion) Conference and Industrial Chain Exhibition

Conference Time: April 23-24, 2024

Conference Venue: Shangri-La Hotel, Suzhou

Conference Address: 168 Tayuan Road, New District, Suzhou City